Nootkatone, the substance behind the citrus flavor and aroma of grapefruit that is already used in beverages and prestige perfumes, may be heading for a new use in battling mosquitoes, ticks and other pests thanks to a less expensive way of making large amounts of the once rare and pricey ingredient.

Nootkatone previously had to be harvested from tons of grapefruit and is an excellent example of the potential for developing new pesticides based on natural sources. Nootkatone is a component of the oil in grapefruit, and has been on the U.S. Food and Drug Administration's list of substances generally recognized as safe for use in food.

It has been in commercial use for years as a flavoring for foods and beverages and as a fragrance ingredient in perfumes. Those applications require only tiny amounts of nootkatone, and price — $25 per ounce when extracted from grapefruit — was not a major concern. It was slightly less expensive when produced from a substance called valencene, extracted from oranges. .

"A new product based on nootkatone would have multiple advantages over existing mosquito repellants based on DEET," said Richard Burlingame, Ph.D.,  of Allylix, Inc., a renewable-chemical firm in Lexington, Ky., who presented the report at the meeting of the American Chemical Society in Indianapolis. "Nootkatone is a broad-spectrum ingredient that has been shown to be effective as a control agent for mosquitoes, ticks and bedbugs. Nootkatone has been used for years to give beverages a grapefruit flavor. It is safe to eat, has a pleasant citrus flavor, is not greasy, both repels and kills insects, and should not have the toxicity concerns that exist for DEET."

The need for a more economical source of nootkatone intensified after scientists at the U.S. Centers for Disease Control and Prevention (CDC) discovered nootkatone's effectiveness in controlling ticks, mosquitoes and other insects. Nootkatone extracted from grapefruit, however, would be too expensive for development of a consumer product. That use would require larger amounts of nootkatone. Allylix is now working with scientists at CDC to develop nootkatone for commercial use as an insect-control agent.

Burlingame described how Allylix used proprietary technology to develop a way of producing valencene from yeast growing in industrial fermentation vats. Technicians harvest the valencene and use a chemical process to convert it into nootkatone. Allylix said the process made it possible to market nootkatone at a competitive price.

"The effects of nootkatone last much longer than those of repellents currently on the market," he said. "And nootkatone shows promise for being the most effective agent for the ticks that cause Lyme disease."

Nookatone also works in a new way, so it can be used against insects that develop resistance and shrug off conventional pesticides, and yet would be very unlikely to harm people or pets.

Allylix currently sells nootkatone only for use in flavor and fragrance applications. The next step involves getting approval from the U.S. Environmental Protection Agency to sell nootkatone for insect control. "They haven't approved it yet, so no products currently on the market in the U.S. include nootkatone as an active ingredient to control pests," noted Burlingame. "But in the future, it could be a key ingredient in repellents for use on clothing or on skin as a spray, or even as a soap or shampoo."

 The scientists received funding from the National Science Foundation through the SBIR program and the Department of Energy. 

A press conference on this topic will be held Wednesday today at 10:45 AM Pacific. Off-site reporters can access live audio and video of the event and ask questions at http://www.ustream.tv/channel/acslive.